ThreatLocker has secured US$190 million in Series F funding to accelerate development of its Zero Trust cyber security platform, strengthen protections against AI-related threats, and expand its international footprint.
The investment round was led by venture capital firm Elephant, with continued backing from D. E. Shaw Ventures and Arthur Ventures, alongside a new investment from Koch Disruptive Technologies.
The funding will also support the opening of a new UK office in Reading as the company expands across Europe.
ThreatLocker said the investment comes amid growing demand for zero trust security as organisations seek to defend against AI-enabled attacks and manage the increasing use of AI agents within enterprise environments.
Chief executive and co-founder Danny Jenkins said the company’s approach differs from traditional cyber security tools by preventing unauthorised activity rather than detecting attacks after they occur.
“Most cyber security tools are still built around identifying malicious activity after it has already entered an environment, when the damage may already be done,” Jenkins (pictured) said in a statement.
“We believe the better model is to define what is allowed and deny everything else by default.”
The company said it will continue developing capabilities to manage AI-related risks, including application allowlisting to prevent unauthorised AI tools and AI-generated code from executing, and its Ringfencing technology, which restricts what approved applications and AI agents can access and modify.
Elephant partner Jeremiah Daly said the company had established itself as a leader in prevention-based cyber security.
“As more organisations seek prevention-based security and practical approaches to zero trust, ThreatLocker is uniquely positioned to capture that demand,” Daly said.
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